mek1 2 (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology mek1 2
Mek1 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mek+1+2/MEK-1%2F2+Antibody/pmc12982509-356-16-26
Average 93 stars, based on 105 article reviews

mek1 2 - by Bioz Stars, 2026-10

93/100 stars

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Incubation:

Article Title: DUS4L suppresses invasion and metastasis in LUAD via modulation of PI3K/AKT and ERK/MAPK signaling through GRB2.
Article Snippet: .. The protein was separated, transferred, and then incubated with 5 % milk for 2 h. Antibodies including DUS4L (Abcam, ab107726), PI3K (Cell Signaling Technology, CST, 4249), p-PI3K p85 alpha (Thermo Fisher Scientific, PA5-104853), AKT (CST, 4691), p-AKT (CST, 4060), mTOR (CST, 2983), p-mTOR (CST, 5536), GRB2 (Santa Cruz, sc-8034), KRAS (Santa Cruz, sc-30), ERK (Santa Cruz, sc-514302), p-ERK (Santa Cruz, sc-81492), MEK-1/2 (Santa Cruz, sc-81504), p-MEK-1/2 (Santa Cruz, sc-81503), E-cadherin (CST, sc-8426), N-cadherin (CST, sc59987), Vimentin (CST, sc-6260), β-Tubulin (Proteintech, 11224–1- AP), Flag (ABclonal, AE024), and IgG (Abcam, ab313801) were incubated with the protein overnight at 4 ◦C. .. Following the initial incubation, the membranes were exposed to a secondary antibody (Proteintech, SA00001-1; SA00001-2) conjugated with HRP for 1 h at room temperature.

Membrane:

Article Title: Mouse Spexin: (III) Differential Regulation by Glucose and Insulin in Glandular Stomach and Functional Implication in Feeding Control
Article Snippet: After insulin treatment, gastric mucosal cells were lysed in RIPA buffer containing a cocktail of protease inhibitors and phosphatase inhibitors (Roche). .. The cell lysate prepared was resolved by SDS-PAGE, transblotted onto a nitrocellulose membrane using a TE77 Semi-dry Electroblotting Unit (Asmersham), and subjected to Western blot using the antisera raised against the phosphorylated form (as “P-” form) and total protein (as “T-” form) of insulin receptor (InsR, 1:2000, Abcam), IGF-I receptor (IGF1R, 1:2000, Santa Cruz), MEK 1/2 (1:1000, Cell Signaling), ERK 1/2 (1:5000, Sigma), P 38 MAPK (1:1000, Cell Signaling), PI3K (1:2000, Cell Signaling) and Akt (1:1500, Cell Signaling), respectively. ..

Western Blot:

Article Title: Development and Characterization of an Avirulent Leishmania major Strain.
Article Snippet: .. Western blot Abs for p-PKCd and p-PKCz/l (Cell Signaling Technology, Danvers, MA); pan-Ras (Pierce, Rockford, IL); p-PKCb1, PKCb1, p-PKCd, PKCd, p-PKCz, p-PKCz/l, p-p38MAPK, p38MAPK, p–ERK-1/2, ERK-1/2, p-Lyn, Lyn, p-Syk, Syk, p-PI3k, PI3k, p-Raf, Raf, p–MKP-1, p–MKP-3, p–SHP-1, p–MEK-1/2, MEK-1/2, p–MKK-3/6, MKK-3/6, Ras GTPase, CD40, b-actin, and Luminol reagent were from Santa Cruz Biotechnology (Santa Cruz, CA). .. Anti-cytokine Abs (IL-10, IL-12, IFN-g, and IL-4), cytokine standard for ELISA, anti-CD40 Ab (no azide/low endotoxin; clone 3/23), and isotype control (rat IgG2ak) were purchased from BD Biosciences (San Diego, CA).

Article Title: Signal Transduction for TNFα-Induced Type II SOCS Expression and Its Functional Implication in Growth Hormone Resistance in Carp Hepatocytes
Article Snippet: .. To test if TNFα can induce IKK/NF-κB, JAK/STAT, MAPK, and PI3K/Akt activation in the carp liver, Western blot was performed in cell lysate prepared from carp hepatocytes with TNFα treatment using the antibodies for phosphorylated form (“p-” form) and total protein (“t-” form) of IκBα (1:1,000; Santa Cruz), MEK 1/2 (1:1,000; Cell Signaling), Erk 1/2 (1:5,000; Sigma), P 38 MAPK (1:1,000; Cell Signaling), Akt (1:1,000, Cell Signaling), JAK 2 (1:1,000; Santa Cruz), STAT 1 (1:1,000; Cell Signaling), STAT 3 (1:1,000; Cell Signaling) and STAT 5 (1:1,000; Cell Signaling), respectively. .. In these studies, parallel blotting of β actin with an Actin Ab-1 Kit (Calbiochem) was used as the internal control.

Activation Assay:

other:

Article Title: Artematrolide A inhibited cervical cancer cell proliferation via ROS/ERK/mTOR pathway and metabolic shift.
Article Snippet: Background: Artematrolide A (AR-A), a guaianolide dimer isolated from Artemisia atrovirens, demonstrated significant inhibitory effect on three human hepatoma cell lines (HepG2, Huh7 and SMMC7721).. The anti-cervical cancer effect and mechanism of this compound have yet to be explored.. This study is to reveal the role and mechanisms of artematrolide A on cervical cancer cells, and provide the pharmacological understanding of

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